Liquid Chromatographic Characterization of TNT in Eisenia fetida Liquid Chromatographic Characterization of 2,4,6- Trinitrotoluene Bound Residues Extracted from Eisenia fetida

The nitroaromatic compound TNT is a common component in military munitions because of its chemical stability and comparatively safe method of manufacture. In living systems, TNT is rapidly metabolized into four identifi able and extractable compounds (2-amino-4,6dinitrotoluene, 4-amino-2,6-dinitrotoluene, 2,4-diamino-6-nitrotoluene, and 2,6-diamino-4-nitrotoluene). Exposing model organisms to TNT forms unextractable biotransformed products, commonly called “bound residues.” This project evaluates methods for removal of bound residues from tissue. Liquid chromatography used in combination with radiotracer analysis will provide preliminary insight into the chemical structure(s) of these extractable bound residues.